High Content Analysis for Real-Time Cellular Imaging

OTRADI has the capacity to do high-content imaging of cellular systems in a 96-well format, optimized for automated imaging. We have the capabilities to:

• Design and validate image-based cell screening assays
• Develop and test mechanism of action (MOA) hypotheses
• Simultaneously screen for and prioritize hits based on specificity for target and lack of cytotoxicity
• Provide imaging and analysis of live and fixed cells
• Identify cellular targets for drugs
• Identify and profile enzymatic targets (kinases, proteases, phosphatases, etc.
• Run kinetic assays to image the same cells over an extended time course
• Conduct high-throughput analysis of fixed cells
• Analyze cell morphology and sub-cellular localization/compartmentalization
• Conduct assays designed for counting nuclei, cell scoring (live/dead), analyzing cell health and granularity, as well as determining mitotic index, neurite outgrowth, transfluorescence, translocation, and multi-wavelength cell scoring

We are also interested in working with researchers to develop new applications for high-content analysis, so if you have an idea please do not hesitate to contact our senior scientists.