Otradi
Oregon Translational Research and Drug Development Institute (OTRADI)
OTRADI has invested in state of the art high-content and high-throughput automation machines to facilitate research and development in a timely and cost-efficient manner. Additionally, our scientists have extensive training on these systems, and can advise and help you or your company with custom experimental designs to meet your individual needs.
To find out about a particular system in our lab, follow the links below. If you have questions about any particular piece of equipment, please do not hesitate to contact one of our senior scientists.
- Staccato Automated System from Caliper Life Sciences
- Opera Automated System from Caliper LS
- Additional Equipment
High-throughput screening using the Staccato Automated System from Caliper Life Sciences
Liquid handling robotic workstation: Sciclone ALH3000
This highly flexible, automated liquid handling workstation for drug discovery is configured to handle any automated, liquid-handling-application focus area including genomics, proteomics, immunoassays and cell-based assays. Designed for precision pipetting, it features a high-volume head (1-200uL) and low-volume head (nL) for liquid sampling in 24-, 48-, 96-, and 384-well microplates.
Liquid handler: Sciclone Z-8 liquid handler
This liquid handler is designed for serial dilution, hit picking, control addition, sample transfer and other applications where there is individual and/or independently controlled access to specific single wells or groups of wells in 24-, 48-, 96- and 384-well microplates.
Microplate gripper: Sciclone ALH servo gripper
The gripper stacks and un-stacks plates, unloads stackers and other plate-presentation devices, loads nested tips and de-lids plates.
Microplate and tip handler: HyperStak automated plate-and-tip-storage device and dispenser
This handler stores and dispenses microplates and disposable tips for the Sciclone system.
Robotic microplate handler: Twister II microplate handler
The heart of a completely-automated, integrated assay system, this robotic handler accesses the liquid-handling deck of the Sciclone, as well as the incubator, plate reader, and plate washer/dispenser.
Microplate washer/dispenser: BioTek 405 microplate washer/dispenser
Dual-action manifold gives independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection in both 96- and 384-well microplates.
Barcode reader: Microscan MS-3
Embedded compact barcode scanner for microplate database management.
Microplate incubator: Cytomat 2C incubator
Robot-accessible, 42-microplate position incubator, featuring temperature, humidity, and gaseous atmosphere control for all automated, cell-based and non-cell-based assay systems.
Microplate reader: BioTek Synergy 4 microplate reader
As the most advanced multidetection microplate reader, this model is designed to address the widest range of microplate-based applications. Its filter-based system is designed to allow detection of fluorescence (mercury lamp and laser), luminescence and optical density.
Software and control: Caliper iLinkPro
Caliper Life Sciences' system integration software for assay development, method-building, assay-editing and scheduling, is used to create automated assays, track materials, manage data and run complete systems.
Training: Certified training at Caliper Training Center in Boston, Mass.
OTRADI scientists have received certified training on the Caliper Staccato Automated System and associated software and database management at the Caliper Life Sciences headquarters.
For more information, see www.caliperls.com/assets/009/5934.pdf.
High-content screening and analysis using the OPERA LX HCA System from PerkinElmer
Opera™ LX for confocal microscopy
- Fully automated
- Spinning disk (Yokogawa) confocal microscopy
- Laser autofocusing
- Objectives: (dry) 10X, 20X; (water immersion) 20X, 40X, & 60X.
- Excitation lasers (confocal): 488 nm, 561 nm & 640 nm
- UV excitation (non-confocal): Polychrome V: 360 – 410 nm
- Batch processing: up to 45 microplates (96-well or 384-well)
Acapella™ for image processing & analysis
- Analysis of images from Opera or any other sources
- Standard image-based assays, measuring intensity changes, translocation, & morphology
- Custom image analysis algorithms development
- High performance computation using parallel processing
- Fully automated batch processing
Panmo™ for dynamic graphics based data exploration
- End-user driven interactive exploration of high-dimensional cell-level data
- Cross examination of numerical and image data
For more information, click here.
Additional Equipment
BioTek Precision X2 microplate sampler
Robotic liquid handler and microplate sampler capable of allocating precise volumes of liquid into multiple microplates on platform.
BioTek Bio-Stack microplate stacker
Robotic microplate stacker stores and dispenses microplates to a microplate sampler for increased handling capacity.
Microplate washer/dispenser: BioTek 405 microplate washer/dispenser
Dual-action manifold gives independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection in both 96- and 384-well microplates.
Turner Biosystems Modulus microplate reader
96-well microplate reader combines a luminometer, spectrophotometer, and fluorometer capabilities into one easy-to-use system.
OTRADI's equipment inventory also includes biosafety cabinets, incubators for cell culture, bacterial incubators, spectrophotometer, and a Milli-Q water purification system.